![]() Due to the complexity of the amino acid (AA) alphabet, rich post-translational modification, and diverse subcellular localization of proteins, few versatile tools are available for effective identification and visualization of protein motifs. ![]() Sequence logos have been widely used as graphical representations of conserved nucleic acid and protein motifs. ![]() The significance level was set at 0.05 for all tests. For yeast NatA substrate specificity analyses, the same set of subsequences of 25 residues from the N-termini of the 285 NatA substrates was used as the input set, while background models for Fisher’s exact test and Z-test were built from all subsequences and from randomly sampled subsequences of 25 AA residues from the N-termini of the yeast proteins not including the 285 NatA substrates, respectively. For human GRB substrate specificity analyses, the same set of 416, equal-length subsequences of 30 residues centered on the cleavage sites was used as the input set, while background models for Fisher’s exact test and Z-test were built from all subsequences and from randomly sampled subsequences of 30 AA residues from the UniProt human reference proteome, respectively. Fisher’s exact test ( A and C) and Z-test ( B and D) were performed to identify substrate sequence preferences of human GRB and yeast NatA, with a significance level of 0.05. S3 Fig: dagLogos resulting from different statistical test methods.
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